A NR2E1-interacting peptide of LSD1 inhibits the proliferation of brain tumour initiating cells.

ABSTRACT:

Objectives

Elimination of brain tumour initiating cells (BTICs) is important for the good prognosis of malignant brain tumour treatment. To develop a novel strategy targeting BTICs, we studied NR2E1(TLX) involved self-renewal mechanism of BTICs and explored the intervention means.

Materials and methods

NR2E1 and its interacting protein-LSD1 in BTICs were studied by gene interference combined with cell growth, tumour sphere formation, co-immunoprecipitation and chromatin immunoprecipitation assays. NR2E1 interacting peptide of LSD1 was identified by Amide Hydrogen/Deuterium Exchange and Mass Spectrometry (HDX-MS) and analysed by in vitro functional assays. The in vivo function of the peptide was examined with intracranial mouse model by transplanting patient-derived BTICs.

SUBMITTER: Hu R 

PROVIDER: S-EPMC9816925 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature