Regulatory role of human fibrocartilage stem cells in condyle osteochondroma.

ABSTRACT:

Objective

Osteochondroma is a common benign skeletal disorder for which different molecular and histological features of long bones have been reported. We investigated cell-of-origin and molecular mechanisms of a rare condylar osteochondroma (CO).

Methods

Human fibrocartilage stem cells (hFCSCs) isolated from CO and normal condyle tissue were used for RNA sequencing, real-time PCR, Western Blotting, immunohistology, flowcytometry, as well as for chondrogenic differentiation, proliferation, and apoptosis detection assays.

Results

HFCSCs were fewer in number with weaker proliferative capacity and higher apoptosis ratio in the CO group. During the chondrogenic inducing process, hFCSCs from CO were prone to form more mature and hypertrophic cartilage. The result of RNA se

SUBMITTER: Yin Q 

PROVIDER: S-EPMC9816926 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature