Remodelling landscape of tissue-engineered bladder with porcine small intestine submucosa using single-cell RNA sequencing.

ABSTRACT:

Objective

Bioscaffolds are widely used for tissue engineering, but failed and inconsistent preclinical results have hampered the clinical use of bioscaffolds for tissue engineering. We aimed to construct a cellular remodelling landscape and to identify the key cell subpopulations and important genes driving bladder remodelling.

Methods

Twenty-four reconstructed mouse bladders using porcine small intestinal submucosa (PSIS) were harvested at 1, 3, and 6 weeks to perform single-cell RNA sequencing. Cell types were identified and their differentially expressed genes (DEGs) at each stage were used for functional analysis. Immunofluorescence was used to validate the specific cell type.

Results

The remodelling landscape included 13 cell types. Among them, fibroblasts, smoot

SUBMITTER: Peng L 

PROVIDER: S-EPMC9816928 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature