N⁶ -methyladenosine-modified circRNA RERE modulates osteoarthritis by regulating β-catenin ubiquitination and degradation.

ABSTRACT:

Objectives

N⁶ -methyladenosine (m6A) is one of the most abundant internal RNA modifications. We investigated the role of m6A-modified circRERE in osteoarthritis (OA) and its mechanism.

Materials and methods

CircRERE and IRF2BPL were screened by microarrays. The role of m6A-modification in circRERE was examined by methylated RNA precipitation and morpholino oligo (MOs) treatment. The axis of circRERE/miR-195-5p/IRF2BPL/β-catenin was determined using flow cytometry, western blotting and immunofluorescence in human chondrocytes (HCs) and corroborated using a mouse model of destabilization of medial meniscus (DMM) with intra-articular (IA) injection of adeno-associated viruses (AAV).

Results

CircRERE was decreased in OA cartilage and chondrocytes compared with

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9816929 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature