The effect of membrane thickness on the membrane permeabilizing activity of the cyclic lipopeptide tolaasin II.

ABSTRACT: Tolaasin II is an amphiphilic, membrane-active, cyclic lipopeptide produced by Pseudomonas tolaasii and is responsible for brown blotch disease in mushroom. To better understand the mode of action and membrane selectivity of tolaasin II and related lipopeptides, its permeabilizing effect on liposomes of different membrane thickness was characterized. An equi-activity analysis served to distinguish between the effects of membrane partitioning and the intrinsic activity of the membrane-bound peptide. It was found that thicker membranes require higher local peptide concentrations to become leaky. More specifically, the mole ratio of membrane-bound peptide per lipid needed to induce 50% leakage of calcein within 1 h, R_e ⁵⁰, increased monotonically with membrane thi

SUBMITTER: Steigenberger J 

PROVIDER: S-EPMC9817028 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature