Zwitterionic betaines over HEPES as the new generation biocompatible pH buffers for cell culture.

ABSTRACT: Good's buffers have been widely applied in cell/organ culture over the past half a century as biocompatible pH stabilizers. However, the emergence of severe adverse effects, such as cellular uptake, lysosomal autophagic activation, and visible light-induced cytotoxicity, raises serious questions over its biocompatibility while underlying mechanism was unclear. Here we report that riboflavin (RF, component of cell culture medium) generates ¹O₂, ·OH, and O₂ ^•- under visible light exposure during regular cell manipulation. These short half-life reactive oxygen species (ROS) react with tertiary amine groups of HEPES, producing 106.6 μM of H₂O₂. Orders of magnitude elevated half-life of ROS in the medium caused severe cytotoxicit

SUBMITTER: Liu P 

PROVIDER: S-EPMC9817164 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature