Assignment of aromatic side-chain spins and characterization of their distance restraints at fast MAS.

ABSTRACT: The assignment of aromatic side-chain spins has always been more challenging than assigning backbone and aliphatic spins. Selective labeling combined with mutagenesis has been the approach for assigning aromatic spins. This manuscript reports a method for assigning aromatic spins in a fully protonated protein by connecting them to the backbone atoms using a low-power TOBSY sequence. The pulse sequence employs residual polarization and sequential acquisitions techniques to record H^N- and H^C-detected spectra in a single experiment. The unambiguous assignment of aromatic spins also enables the characterization of ¹H-¹H distance restraints involving aromatic spins. Broadband (RFDR) and selective (BASS-SD) recoupling sequences were used to generate H<

SUBMITTER: Ahlawat S 

PROVIDER: S-EPMC9817166 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature