A change point analysis protocol for comparing intracellular transport by different molecular motor combinations.

ABSTRACT: Intracellular transport by microtubule-based molecular motors is marked by qualitatively different behaviors. It is a long-standing and still-open challenge to accurately quantify the various individual-cargo behaviors and how they are affected by the presence or absence of particular motor families. In this work we introduce a protocol for analyzing change points in cargo trajectories that can be faithfully projected along the length of a (mostly) straight microtubule. Our protocol consists of automated identification of velocity change points, estimation of velocities during the behavior segments, and extrapolation to motor-specific velocity distributions. Using simulated data we show that our method compares favorably with existing methods. We then apply the technique to data sets in wh

SUBMITTER: Jensen MA 

PROVIDER: S-EPMC9817212 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature