"Proteotranscriptomic analysis of advanced colorectal cancer patient derived organoids for drug sensitivity prediction".

ABSTRACT:

Background

Patient-derived organoids (PDOs) from advanced colorectal cancer (CRC) patients could be a key platform to predict drug response and discover new biomarkers. We aimed to integrate PDO drug response with multi-omics characterization beyond genomics.

Methods

We generated 29 PDO lines from 22 advanced CRC patients and provided a morphologic, genomic, and transcriptomic characterization. We performed drug sensitivity assays with a panel of both standard and non-standard agents in five long-term cultures, and integrated drug response with a baseline proteomic and transcriptomic characterization by SWATH-MS and RNA-seq analysis, respectively.

Results

PDOs were successfully generated from heavily pre-treated patients, including a paired model of advanced MSI high

SUBMITTER: Papaccio F 

PROVIDER: S-EPMC9817273 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature