Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle.

ABSTRACT:

Background

Several fusion tags for separation handle have been developed, but the fused tag for simply and cheaply separating the target protein is still lacking.

Results

Separation conditions for the human annexin A1 (hanA1) tagged emerald green fluorescent protein (EmGFP) in Escherichia coli were optimized via precipitation with calcium chloride (CaCl₂) and resolubilization with ethylenediamine tetraacetic acid disodium salt (EDTA-Na₂). The HanA1-EmGFP absorbing with other three affinity matrix was detected, only it was strongly bound to heparin Sepharose. The separation efficiency of the HanA1-EmGFP was comparable with purification efficiency of the His6-tagged HanA1-EmGFP via metal ion affinity chromatography. Three fluorescent proteins for the EmGF

SUBMITTER: He X 

PROVIDER: S-EPMC9817314 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature