Targeting fatty acid synthase modulates sensitivity of hepatocellular carcinoma to sorafenib via ferroptosis.

ABSTRACT:

Background

Sorafenib resistance is a key impediment to successful treatment of patients with advanced hepatocellular carcinoma (HCC) and recent studies have reported reversal of drug resistance by targeting ferroptosis. The present study aimed to explore the association of fatty acid synthase (FASN) with sorafenib resistance via regulation of ferroptosis and provide a novel treatment strategy to overcome the sorafenib resistance of HCC patients.

Methods

Intracellular levels of lipid peroxides, glutathione, malondialdehyde, and Fe²⁺ were measured as indicators of ferroptosis status. Biological information analyses, immunofluorescence assays, western blot assays, and co-immunoprecipitation analyses were conducted to elucidate the functions of FASN in HCC. Both in vi

SUBMITTER: Li Y 

PROVIDER: S-EPMC9817350 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature