Firocoxib as a Potential Neoadjuvant Treatment in Canine Patients with Triple-Negative Mammary Gland Tumors.

ABSTRACT: This study aimed to investigate the pro-apoptotic effects of NSAID (Previcox^®) in vitro and in vivo. Two CMT cell lines, one from the primary tumor and one from bone metastasis, were treated with firocoxib and MTT assay was performed to determine the half-maximal inhibitory concentration (IC₅₀) value. The firocoxib IC₅₀ for the cell lines UNESP-CM5 and UNESP-MM1 were 25.21 µM and 27.41 µM, respectively. The cell lines were then treated with the respective firocoxib IC₅₀ concentrations and annexin V/propidium iodide (PI) assay was performed, to detect the induction of apoptosis in both cells (Annexin+/PI+). We conducted an in vivo study involving female dogs affected by CMT and divided them into control and treatment groups. For both groups, a bio

SUBMITTER: Brandi A 

PROVIDER: S-EPMC9817520 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature