Long-Term In Vitro Maintenance of Piglet Testicular Tissue: Effects of Tissue Fragment Size, Preparation Method, and Serum Source

ABSTRACT:

Simple Summary

One out of every 650 children is affected by cancer, and about half of survivors become permanently infertile due to the side effects of cancer treatments. Pre-treatment testicular biopsies can be collected and cryopreserved for future use to uphold biological fatherhood. Currently, the only safe approach to produce haploid germ cells is through in vitro spermatogenesis (IVS), which has only been fully achieved in mice. Pigs are a more suitable animal model for the study of testicular development and IVS, not only because of their anatomical and physiological similarities to humans but also their long prepubertal period. This study explored optimal conditions for the long-term culture of neonatal porcine testicular tissues as a first step in achieving IVS from pigs,

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PROVIDER: S-EPMC9817678 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature