Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing.

ABSTRACT:

Background

The placenta is an important organ for fetal and maternal health during pregnancy and impacts offspring health late in life. Defects in placental vasculature and trophoblast have been identified in several pregnancy complications. Thus, the detailed molecular profile and heterogeneity of endothelial cells and trophoblasts in placentas will aid us in better understanding placental behaviors and improving pregnancy outcomes.

Methods

Single-cell RNA sequencing (scRNA-seq) was performed to profile the transcriptomics of human placental villous tissues from eleven patients with normal pregnancies in the first and second trimesters (6-16 weeks of gestation).

Results

The transcriptomic landscape of 52,179 single cells was obtained, and the cells were classified as

SUBMITTER: Li H 

PROVIDER: S-EPMC9818681 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature