A Novel Method for Primary Blood Cell Culturing and Selection in Drosophila melanogaster.

ABSTRACT: The blood cells of the fruit fly Drosophila melanogaster show many similarities to their vertebrate counterparts, both in their functions and their differentiation. In the past decades, a wide palette of immunological and transgenic tools and methods have been developed to study hematopoiesis in the Drosophila larva. However, the in vivo observation of blood cells is technically restricted by the limited transparency of the body and the difficulty in keeping the organism alive during imaging. Here we describe an improved ex vivo culturing method that allows effective visualization and selection of live blood cells in primary cultures derived from Drosophila larvae. Our results show that cultured hemocytes accurately represent morphological and functional changes follow

SUBMITTER: Kuthy-Sutus E 

PROVIDER: S-EPMC9818912 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature