Mechanism of Oxytocin-Induced Contraction in Rat Gastric Circular Smooth Muscle.

ABSTRACT: Oxytocin produces an excitatory effect on gastric muscle through the activation of receptors present on stomach smooth muscle cells. However, the intracellular mechanisms that mediate oxytocin excitatory effects are still largely unknown. Therefore, we aimed to investigate the signaling pathways involved in oxytocin-induced contractions in gastric smooth muscle, shedding light on phospholipase C (PLC)-β1 signaling and its downstream molecules, including inositol 1,4,5- trisphosphate (IP₃) and myosin light chain kinase (MLCK). The contractions of gastric smooth muscle from male rats were measured in an organ bath set up in response to exogenous oxytocin 10^-7 M, in the presence and absence of inhibitors of the indicated signaling molecules. Oxytocin (10^-9-10<

SUBMITTER: Alqudah M 

PROVIDER: S-EPMC9820280 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature