Protein-Coding Region Derived Small RNA in Exosomes from Influenza A Virus-Infected Cells.

ABSTRACT: Exosomes may function as multifactorial mediators of cell-to-cell communication, playing crucial roles in both physiological and pathological processes. Exosomes released from virus-infected cells may contain RNA and proteins facilitating infection spread. The purpose of our study was to analyze how the small RNA content of exosomes is affected by infection with the influenza A virus (IAV). Exosomes were isolated by ultracentrifugation after hemadsorption of virions and their small RNA content was identified using high-throughput sequencing. As compared to mock-infected controls, 856 RNA transcripts were significantly differentially expressed in exosomes from IAV-infected cells, including fragments of 458 protein-coding (pcRNA), 336 small, 28 long intergenic non-coding RNA transcripts, and

SUBMITTER: Kwasnik M 

PROVIDER: S-EPMC9820831 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature