Label-Free Characterization of Macrophage Polarization Using Raman Spectroscopy.

ABSTRACT: Macrophages are important cells of the innate immune system that play many different roles in host defense, a fact that is reflected by their polarization into many distinct subtypes. Depending on their function and phenotype, macrophages can be grossly classified into classically activated macrophages (pro-inflammatory M1 cells), alternatively activated macrophages (anti-inflammatory M2 cells), and non-activated cells (resting M0 cells). A fast, label-free and non-destructive characterization of macrophage phenotypes could be of importance for studying the contribution of the various subtypes to numerous pathologies. In this work, single cell Raman spectroscopic imaging was applied to visualize the characteristic phenotype as well as to discriminate between different human macrophage phen

SUBMITTER: Naumann M 

PROVIDER: S-EPMC9821063 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature