The study of GSDMB in pathogenesis of psoriasis vulgaris.

ABSTRACT:

Background

Gasdermin (GSDM) B is a member of the GSDM family, which is a protein that may be involved in the cell pyroptosis process and is associated with inflammatory diseases.

Objective

To explore the correlation between GSDMB and psoriasis vulgaris.

Methods

Skin lesions from 33 patients with psoriasis vulgaris and 69 normal controls were collected. ELISA and Western blot were adopted to detect proteins. The HaCaT cell line was transfected with 3 sets of interfering sequence siRNA, and the mRNA and protein levels before and after the transfection were measured by qPCR and Western blot respectively, so as to establish a cell model with low GSDMB gene expression; the MTT method was used to detect cells viability, flow cytometry to detect cell apoptosis.

Results

SUBMITTER: Ji X 

PROVIDER: S-EPMC9821418 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature