METTL3-Mediated lncSNHG7 m⁶A Modification in the Osteogenic/Odontogenic Differentiation of Human Dental Stem Cells.

ABSTRACT: Background: Human dental pulp stem cells (hDPSCs) play an important role in endodontic regeneration. N6-methyladenosine (m⁶A) is the most common RNA modification, and noncoding RNAs have also been demonstrated to have regulatory roles in the expression of m⁶A regulatory proteins. However, the study on m⁶A modification in hDPSCs has not yet been conducted. Methods: Single base site PCR (MazF) was used to detect the m⁶A modification site of lncSNHG7 before and after mineralization of hDPSCs to screen the target m⁶A modification protein, and bioinformatics analysis was used to analyze the related pathways rich in lncSNHG7. After knockdown and overexpression of lncSNHG7 and METTL3, the osteogenic/odontogenic ability was detec

SUBMITTER: Yang Y 

PROVIDER: S-EPMC9821659 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature