Single-cell profiling identifies ACE⁺ granuloma macrophages as a nonpermissive niche for intracellular bacteria during persistent Salmonella infection.

ABSTRACT: Macrophages mediate key antimicrobial responses against intracellular bacterial pathogens, such as Salmonella enterica. Yet, they can also act as a permissive niche for these pathogens to persist in infected tissues within granulomas, which are immunological structures composed of macrophages and other immune cells. We apply single-cell transcriptomics to investigate macrophage functional diversity during persistent S. enterica serovar Typhimurium (STm) infection in mice. We identify determinants of macrophage heterogeneity in infected spleens and describe populations of distinct phenotypes, functional programming, and spatial localization. Using an STm mutant with impaired ability to polarize macrophage phenotypes, we find that angiotensin-converting enzyme (AC

SUBMITTER: Pham THM 

PROVIDER: S-EPMC9821941 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature