Exercise blood-drop metabolic profiling links metabolism with perceived exertion.

ABSTRACT: Background: Assessing detailed metabolism in exercising persons minute-to-minute has not been possible. We developed a "drop-of-blood" platform to fulfill that need. Our study aimed not only to demonstrate the utility of our methodology, but also to give insights into unknown mechanisms and new directions. Methods: We developed a platform, based on gas chromatography and mass spectrometry, to assess metabolism from a blood-drop. We first observed a single volunteer who ran 13 km in 60 min. We particularly monitored relative perceived exertion (RPE). We observed that 2,3-bisphosphoglycerate peaked at RPE in this subject. We next expanded these findings to women and men volunteers who performed an RPE-based exercise protocol to RPE at Fi O ₂ 20.9% or Fi

SUBMITTER: Opialla T 

PROVIDER: S-EPMC9822726 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature