Epsin Nanotherapy Regulates Cholesterol Transport to Fortify Atheroma Regression.

ABSTRACT:

Background

Excess cholesterol accumulation in lesional macrophages elicits complex responses in atherosclerosis. Epsins, a family of endocytic adaptors, fuel the progression of atherosclerosis; however, the underlying mechanism and therapeutic potential of targeting Epsins remains unknown. In this study, we determined the role of Epsins in macrophage-mediated metabolic regulation. We then developed an innovative method to therapeutically target macrophage Epsins with specially designed S2P-conjugated lipid nanoparticles, which encapsulate small-interfering RNAs to suppress Epsins.

Methods

We used single-cell RNA sequencing with our newly developed algorithm MEBOCOST (Metabolite-mediated Cell Communication Modeling by Single Cell Transcriptome) to study cell-cell communicatio

SUBMITTER: Cui K 

PROVIDER: S-EPMC9822875 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature