Stability of sample extracts of vitamin D₃ metabolites after chemical derivatization for LC-MS/MS analysis.

ABSTRACT: Liquid chromatography/tandem mass spectrometry (LC-MS/MS) is widely used to determine vitamin D₃ metabolites in biological samples. The ionization efficiencies of these metabolites, however, are poor under electrospray ionization conditions. Moreover, the chromatographic separation of multiple vitamin D metabolites and their epimers can be challenging. For these reasons, chemical derivatization reagents are often used to improve sensitivity and selectivity of analysis. While the derivatization schemes have been proven to be very effective, one missing aspect is the investigation of the stability of the chemical derivatization products in stored sample extracts. In this study, we investigated the long-term stability of several vitamin D₃ metabolites after 1 and 3 month

SUBMITTER: Alexandridou A 

PROVIDER: S-EPMC9823060 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature