Interplay between Cruciferous Vegetables and the Gut Microbiome: A Multi-Omic Approach.

ABSTRACT: Brassica vegetables contain a multitude of bioactive compounds that prevent and suppress cancer and promote health. Evidence suggests that the gut microbiome may be essential in the production of these compounds; however, the relationship between specific microbes and the abundance of metabolites produced during cruciferous vegetable digestion are still unclear. We utilized an ex vivo human fecal incubation model with in vitro digested broccoli sprouts (Broc), Brussels sprouts (Brus), a combination of the two vegetables (Combo), or a negative control (NC) to investigate microbial metabolites of cruciferous vegetables. We conducted untargeted metabolomics on the fecal cultures by LC-MS/MS and completed 16S rRNA gene sequencing. We identified 72 microbial genera in our samples, 29 of which w

SUBMITTER: Bouranis JA 

PROVIDER: S-EPMC9824405 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature