Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts.

ABSTRACT:

Background

Chloroplast transformation is a robust technology for the expression of recombinant proteins. Various types of pharmaceutical proteins including growth factors have been reported in chloroplasts via chloroplast transformation approach at high expression levels. However, high expression of epidermal growth factor (EGF) in chloroplasts with the technology is still unavailable.

Results

The present work explored the high-level expression of recombinant EGF, a protein widely applied in many clinical therapies, in tobacco chloroplasts. In this work, homoplastic transgenic plants expressing fusion protein GFP-EGF, which was composed of GFP and EGF via a linker, were generated. The expression of GFP-EGF was confirmed by the combination of green fluorescent observation and

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9824920 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature