LncRNA-mRNA expression profile and functional network of vascular dysfunction in septic rats.

ABSTRACT:

Background

We used microarrays to analyse the changes in long non-coding RNAs (lncRNAs) and mRNAs in aorta tissue in model rats with lipopolysaccharide-induced sepsis and determined the lncRNA-mRNA and lncRNA-miRNA-mRNA functional networks.

Methods

Wistar rats were intraperitoneally injected with lipopolysaccharide, and the lncRNA and mRNA expression profiles in the aorta were evaluated using microarrays. The functions of the differentially expressed mRNAs were analysed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. We then constructed coding/non-coding co-expression and competing endogenous RNA networks to study the mechanisms related to sepsis in rats.

Results

We identified 503 differentially expressed lncRNAs and 2479 d

SUBMITTER: Han YC 

PROVIDER: S-EPMC9824925 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature