Differential CpG DNA methylation of peripheral B cells, CD4⁺ T cells, and salivary gland tissues in IgG4-related disease.

ABSTRACT:

Objectives

Immunoglobulin-G4-related disease (IgG4-RD) is a distinct systemic autoimmune-mediated disease manifesting as chronic inflammation and tissue fibrosis. Since the role of DNA methylation in the pathogenesis of IgG4-RD is still unclear, we conduct this study to investigate epigenetic modifications in IgG4-RD.

Methods

A genome-wide DNA methylation study was conducted with B cells, CD4⁺ T cells, and salivary gland tissues from IgG4-RD patients and matched controls by using the Illumina HumanMethylation 850K BeadChip. We further performed pyrosequencing and immunohistochemistry assays to validate the methylation status of some targets of interest.

Results

We identified differentially methylated CpG sites including 44 hypomethylated and 166 hypermethyl

SUBMITTER: Wu X 

PROVIDER: S-EPMC9824958 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature