Combination of automated sample preparation and micro-flow LC-MS for high-throughput plasma proteomics.

ABSTRACT:

Background

Non-invasive detection of blood-based markers is a critical clinical need. Plasma has become the main sample type for clinical proteomics research because it is easy to obtain and contains measurable protein biomarkers that can reveal disease-related physiological and pathological changes. Many efforts have been made to improve the depth of its identification, while there is an increasing need to improve the throughput and reproducibility of plasma proteomics analysis in order to adapt to the clinical large-scale sample analysis.

Methods

We have developed and optimized a robust plasma analysis workflow that combines an automated sample preparation platform with a micro-flow LC-MS-based detection method. The stability and reproducibility of the workflow were system

SUBMITTER: Ye X 

PROVIDER: S-EPMC9824974 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature