Rational design of hybrid DNA-RNA triplex structures as modulators of transcriptional activity in vitro.

ABSTRACT: Triplex nanostructures can be formed in vitro in the promoter region of DNA templates, and it is commonly accepted that these assemblies inhibit the transcription of the downstream genes. Herein, a proof of concept highlighting the possibility of the up- or downregulation of RNA transcription is presented. Hybrid DNA-RNA triplex nanostructures were rationally designed to produce bacterial transcription units with switchable promoters. The rate of RNA production was measured using the signal of a transcribed fluorescent RNA aptamer (i.e. Broccoli). Indeed, several designed bacterial promoters showed the ability of induced transcriptional inhibition, while other properly tailored sequences demonstrated switchable enhancement of transcriptional activity, representing an unprecedented feature

SUBMITTER: Cecconello A 

PROVIDER: S-EPMC9825147 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature