AccuVIR: an ACCUrate VIRal genome assembly tool for third-generation sequencing data.

ABSTRACT:

Motivation

RNA viruses tend to mutate constantly. While many of the variants are neutral, some can lead to higher transmissibility or virulence. Accurate assembly of complete viral genomes enables the identification of underlying variants, which are essential for studying virus evolution and elucidating the relationship between genotypes and virus properties. Recently, third-generation sequencing platforms such as Nanopore sequencers have been used for real-time virus sequencing for Ebola, Zika, coronavirus disease 2019, etc. However, their high per-base error rate prevents the accurate reconstruction of the viral genome.

Results

In this work, we introduce a new tool, AccuVIR, for viral genome assembly and polishing using error-prone long reads. It can better distinguish seq

SUBMITTER: Yu R 

PROVIDER: S-EPMC9825286 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature