Novel cancer gene discovery using a forward genetic screen in RCAS-PDGFB-driven gliomas.

ABSTRACT:

Background

Malignant gliomas, the most common malignant brain tumors in adults, represent a heterogeneous group of diseases with poor prognosis. Retroviruses can cause permanent genetic alterations that modify genes close to the viral integration site.

Methods

Here we describe the use of a high-throughput pipeline coupled to the commonly used tissue-specific retroviral RCAS-TVA mouse tumor model system. Utilizing next-generation sequencing, we show that retroviral integration sites can be reproducibly detected in malignant stem cell lines generated from RCAS-PDGFB-driven glioma biopsies.

Results

A large fraction of common integration sites contained genes that have been dysregulated or misexpressed in glioma. Others overlapped with loci identified in previous glioma-r

SUBMITTER: Weishaupt H 

PROVIDER: S-EPMC9825320 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature