Cab45 deficiency leads to the mistargeting of progranulin and prosaposin and aberrant lysosomal positioning.

ABSTRACT: The trans-Golgi Network (TGN) sorts molecular "addresses" and sends newly synthesized proteins to their destination via vesicular transport carriers. Despite the functional significance of packaging processes at the TGN, the sorting of soluble proteins remains poorly understood. Recent research has shown that the Golgi resident protein Cab45 is a significant regulator of secretory cargo sorting at the TGN. Cab45 oligomerizes upon transient Ca²⁺ influx, recruits soluble cargo molecules (clients), and packs them in sphingomyelin-rich transport carriers. However, the identity of client molecules packed into Cab45 vesicles is scarce. Therefore, we used a precise and highly efficient secretome analysis technology called hiSPECs. Intriguingly, we observed that Cab45 deficient cells ma

SUBMITTER: Tran ML 

PROVIDER: S-EPMC9825660 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature