Epigenome-wide DNA methylation in leukocytes and toenail metals: The normative aging study.

ABSTRACT:

Background

Environmental metal exposures have been associated with multiple deleterious health endpoints. DNA methylation (DNAm) may provide insight into the mechanisms underlying these relationships. Toenail metals are non-invasive biomarkers, reflecting a medium-term time exposure window.

Objectives

This study examined variation in leukocyte DNAm and toenail arsenic (As), cadmium (Cd), lead (Pb), manganese (Mn), and mercury (Hg) among elderly men in the Normative Aging Study, a longitudinal cohort.

Methods

We repeatedly collected samples of blood and toenail clippings. We measured DNAm in leukocytes with the Illumina HumanMethylation450 K BeadChip. We first performed median regression to evaluate the effects of each individual toenail metal on DNAm at three levels:

SUBMITTER: Wang C 

PROVIDER: S-EPMC9825663 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature