Potential of RH5 Antisense on Plasmodium falciparum Proliferation Abatement.

ABSTRACT:

Background

Infections by Plasmodium falciparum, are becoming increasingly difficult to treat. Therefore, there is an urgent need for novel antimalarial agents' discovery against infection. In present study, we described a 2'-O-Methyl gapmer phosphorothioate oligonucleotide antisense targeting translation initiation region of 3D7 strain RH5 gene.

Methods

The study was conducted in Pasteur Institute of Iran in 2020. ODNs effects were measured by microscopic examination and real time RT-PCR. For microscopy, microplates were charged with 2'-OMe ODNs at different dilutions. Unsynchronized parasites were added to a total of 0.4 ml (0.4% parasitemia, 5% red blood cells), and slides were prepared. Proportion of infected cells was measured by counting at least 500 red b

SUBMITTER: Razavi Vakhshourpour S 

PROVIDER: S-EPMC9825705 | biostudies-literature | 2022 Oct-Dec

REPOSITORIES: biostudies-literature