SCExecute: custom cell barcode-stratified analyses of scRNA-seq data.

ABSTRACT:

Motivation

In single-cell RNA-sequencing (scRNA-seq) data, stratification of sequencing reads by cellular barcode is necessary to study cell-specific features. However, apart from gene expression, the analyses of cell-specific features are not sufficiently supported by available tools designed for high-throughput sequencing data.

Results

We introduce SCExecute, which executes a user-provided command on barcode-stratified, extracted on-the-fly, single-cell binary alignment map (scBAM) files. SCExecute extracts the alignments with each cell barcode from aligned, pooled single-cell sequencing data. Simple commands, monolithic programs, multi-command shell scripts or complex shell-based pipelines are then executed on each scBAM file. scBAM files can be restricted to specific bar

SUBMITTER: Edwards N 

PROVIDER: S-EPMC9825775 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature