Regulation of corticotropin-releasing hormone neuronal network activity by noradrenergic stress signals.

ABSTRACT: Noradrenaline is a neurotransmitter released in response to homeostatic challenge and activates the hypothalamic-pituitary-adrenal axis via stimulation of corticotropin-releasing hormone (CRH) neurons. Here we investigated the mechanism through which noradrenaline regulates activity within the CRH neuronal network. Using a combination of in vitro GCaMP6f Ca²⁺ imaging and electrophysiology, we show that noradrenaline induces a robust increase in excitability in a proportion of CRH neurons with many neurons displaying a bursting mode of activity. Noradrenaline-induced activation required α₁ -adrenoceptors and L-type voltage-gated Ca²⁺ channels, but not GABA/glutamate synaptic transmission or sodium action potentials. Exposure of mice to elevated corticosteron

SUBMITTER: Gouws JM 

PROVIDER: S-EPMC9825848 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature