Removal of platelets from blood plasma to improve the quality of extracellular vesicle research.

ABSTRACT:

Background

Blood plasma is commonly used for biomarker research of extracellular vesicles (EVs). Removing all cells prior to analysis of EVs is essential.

Objectives

We therefore studied the efficacy of the most commonly used centrifugation protocol to prepare cell-free plasma.

Methods

Plasma was prepared according to the double centrifugation protocol of the International Society on Thrombosis and Haemostasis (ISTH) in three independent studies. The concentrations of platelets, platelet-derived EVs, and erythrocyte-derived EVs were measured by calibrated flow cytometry.

Results

The mean platelet concentration ranged from 5.1 × 10⁵ /ml to 2.8 × 10⁷ /ml and differed 55-fold between studies. Thus, the ISTH centrifugation protocol does not re

SUBMITTER: Bettin B 

PROVIDER: S-EPMC9825910 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature