THRIFTY: a novel high-throughput method for rapid fibre type identification of isolated skeletal muscle fibres.

ABSTRACT: Fibre type-specific analyses are required for broader understanding of muscle physiology, but such analyses are difficult to conduct due to the extreme time requirements of dissecting and fibre typing individual fibres. Investigations are often confined to a small number of fibres from few participants with low representativeness of the entire fibre population and the participant population. To increase the feasibility of conducting large-scale fibre type-specific studies, a valid and rapid method for high-throughput fibre typing of individually dissected fibres was developed and named THRIFTY (for high-THRoughput Immunofluorescence Fibre TYping). Employing THRIFTY, 400 fibre segments were fixed onto microscope slides with a pre-printed coordinated grid system, probed with antibodies again

SUBMITTER: Horwath O 

PROVIDER: S-EPMC9825974 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature