Ultrasound, as a hypomethylating agent, remodels DNA methylation and alters mRNA transcription in winter wheat (Triticum aestivum L.) seedlings.

ABSTRACT: Any treatment that affects seed germination and seedling development is of paramount importance from an agricultural point of view since they are critical prerequisites for successful crop production. In present study, we have examined the after-effect of ultrasonication (at 30 kHz, 70 W for 5 min) of winter wheat (Triticum aestivum L. cv. SE15) seeds on the early seedling growth and development, and accompanying changes in the DNA methylation and transcriptomic pattern in 7-day-old seedlings. We used mRNA-sequencing and whole genome bisulfite sequencing (WGBS) to identify significantly differentially expressed genes (DEGs), significantly differently methylated regions (DMRs) and genes (DMGs). Ultrasonication of seeds did not alter the germination rate but increased both the length and wei

SUBMITTER: Hidvegi N 

PROVIDER: S-EPMC9826007 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature