Post-Assembly Modification of Protein Cages by Ubc9-Mediated Lysine Acylation.

ABSTRACT: Although viruses have been successfully repurposed as vaccines, antibiotics, and anticancer therapeutics, they also raise concerns regarding genome integration and immunogenicity. Virus-like particles and non-viral protein cages represent a potentially safer alternative but often lack desired functionality. Here, we investigated the utility of a new enzymatic bioconjugation method, called lysine acylation using conjugating enzymes (LACE), to chemoenzymatically modify protein cages. We equipped two structurally distinct protein capsules with a LACE-reactive peptide tag and demonstrated their modification with diverse ligands. This modular approach combines the advantages of chemical conjugation and genetic fusion and allows for site-specific modification with recombinant proteins as well as

SUBMITTER: Levasseur MD 

PROVIDER: S-EPMC9826087 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature