Differences between blood and cerebrospinal fluid glial fibrillary Acidic protein levels: The effect of sample stability.

ABSTRACT:

Introduction

Recent evidence has shown that the marker of reactive astrogliosis, glial fibrillary acidic protein (GFAP), has a stronger relationship with cerebral amyloid beta (Aβ) pathology in blood than in cerebrospinal fluid (CSF). This study investigates if pre-analytical treatment of blood and CSF contribute to these unexpected findings.

Methods

Paired CSF and serum samples from 49 individuals (Aβ-negative = 28; Aβ-positive = 21) underwent a series of seven freeze-thaw cycles (FTCs). All samples were analyzed for GFAP and neurofilament light (NfL) using single molecule array technology including a fresh unfrozen sample from each patient.

Results

FTC significantly affected CSF GFAP concentration (-188.12 pg/ml per FTC) but not serum GFAP. In the same samples, NfL

SUBMITTER: Simren J 

PROVIDER: S-EPMC9826213 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature