A practical and rapid screening method for influenza virus neuraminidase inhibitors based on fluorescence detection.

ABSTRACT: A new analytical method for rapid screening of influenza virus neuraminidase inhibitors was established. The method is based on the principle that, given a certain amount of neuraminidase, the sample and the neuraminidase act in the microplate for a period of time, and the active neuraminidase that is not inhibited by the sample can generate a fluorescence value at a specific wavelength after binding to the substrate, and the rate of inhibition of neuraminidase by the sample can be calculated based on the actual detected fluorescence value. This newly developed method was used to screen and evaluate the in vitro anti-neuraminidase activity of 39 high-purity compounds contained in three traditional Chinese herbal medicines, and finally 25 compounds with strong activity were obtained. The ne

SUBMITTER: Xie J 

PROVIDER: S-EPMC9826620 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature