The Ewald sphere/focus gradient does not limit the resolution of cryoEM reconstructions.

ABSTRACT: In our quest to solve biomolecular structures to higher resolutions in cryoEM, care must be taken to deal with all aspects of image formation in the electron microscope. One of these is the Ewald sphere/focus gradient that derives from the scattering geometry in the microscope and its implications for recovering high resolution and handedness information. While several methods to deal with it has been proposed and implemented, there are still questions as to the correct approach. At the high acceleration voltages used for cryoEM, the traditional projection approximation that ignores the Ewald sphere breaks down around 2-3 Å and with large particles. This is likely not crucial for most biologically interesting molecules, but is required to understand detail about catalytic events, molecular

SUBMITTER: Heymann JB 

PROVIDER: S-EPMC9826812 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature