Characterization of diverse populations of sinoatrial node cells and their proliferation potential at single nucleus resolution.

ABSTRACT:

Background

Each heartbeat is initiated in the sinoatrial node (SAN), and although a recent study (GSE130710) using single nucleus RNA-seq had discovered different populations of cell types within SAN tissue, the distinct potential functions of these cell types have not been delineated.

Methods

To infer some special potential functions of different SAN cell clusters, we applied principal component analysis (PCA), t-distributed stochastic neighbor embedding (t-SNE) and uniform manifold approximation and projection (UMAP) to the GSE130710 dataset to reduce dimensions, followed by Pseudotime trajectory and AUCell analyses, ANOVA and Hurdle statistical models, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichments to determine functional potential o

SUBMITTER: Qu JH 

PROVIDER: S-EPMC9826826 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature