Effects of black cumin-based antimalarial drug loaded with nano-emulsion of bovine and human serum albumins by spectroscopic and molecular docking studies.

ABSTRACT: The growing understanding of nanoemulsion biomedical applications necessitates a basic understanding of protein-drug-loaded nanoemulsion interaction. In our present study, we investigated the binding interactions of Mefloquine (MEF)-loaded black cumin seed oil (Thymoquinone) nanoemulsion of different concentrations towards human and bovine serum albumin (HSA&BSA).Fluorescenceemission,three-dimensionalspectra,UV-visible spectroscopy, and FTIR-spectroscopy, techniques were used together with molecular docking studies to identify the binding effects. The ground state complex formation between Mefloquine-loaded black cumin seed oil nanoemulsion and protein fluorophores was confirmed by a decrease in fluorescence intensity and disputed hyper-chronicity found in the UV-visible spectra of albumin

SUBMITTER: Mohapatra P 

PROVIDER: S-EPMC9826829 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature