Protocol to assess arteriovenous differences across the liver and hindlimb muscles in mice following treadmill exercise.

ABSTRACT: The tissue-specific release and uptake of metabolites in response to exercise is incompletely understood. Here, we detail a protocol to assess arteriovenous differences across the liver and hindlimb muscles in response to treadmill exercise in mice. We describe steps for the treadmill running of mice and the region-specific sampling of blood from the liver and hindlimb. This procedure is particularly relevant for the study of tissue-specific metabolism in response to exercise. For complete details on the use and execution of this protocol, please refer to Sato et al. (2022).¹.

SUBMITTER: Ashcroft SP 

PROVIDER: S-EPMC9826965 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature