DEPTOR loss impairs brown adipocyte development in vitro but has limited impacts in mice.

ABSTRACT:

Objectives

The mechanistic target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth and metabolism. In mice, activation of mTOR controls cold adaptation by promoting the recruitment and the activation of brown adipose tissue (BAT). DEP-domain containing mTOR-interacting protein (DEPTOR) interacts with mTOR to modulate its activity. Whether DEPTOR levels are modulated by cold in BAT and whether this protein regulates brown adipocyte development and thermogenic activation has never been tested.

Methods

DEPTOR levels were measured in mouse tissues upon cold exposure and in brown preadipocytes following the induction of adipogenesis. Lentiviruses expressing short-hairpin RNA were used to repress DEPTOR expression in brown preadipocytes in vitro. Conditional

SUBMITTER: Colas C 

PROVIDER: S-EPMC9827061 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature