Dynamic network biomarker factors orchestrate cell-fate determination at tipping points during hESC differentiation.

ABSTRACT: The generation of ectoderm, mesoderm, and endoderm layers is the most critical biological process during the gastrulation of embryo development. Such a differentiation process in human embryonic stem cells (hESCs) is an inherently nonlinear multi-stage dynamical process which contain multiple tipping points playing crucial roles in the cell-fate decision. However, the tipping points of the process are largely unknown, letting alone the understanding of the molecular regulation on these critical events. Here by designing a module-based dynamic network biomarker (M-DNB) model, we quantitatively pinpointed two tipping points of the differentiation of hESCs toward definitive endoderm, which leads to the identification of M-DNB factors (FOS, HSF1, MYCN, TP53, and

SUBMITTER: Li L 

PROVIDER: S-EPMC9827382 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature