OmpR and Prc contribute to switch the Salmonella morphogenetic program in response to phagosome cues.

ABSTRACT: Salmonella enterica serovar Typhimurium infects eukaryotic cells residing within membrane-bound phagosomes. In this compartment, the pathogen replaces the morphogenetic penicillin-binding proteins 2 and 3 (PBP2/PBP3) with PBP2_SAL /PBP3_SAL , two proteins absent in Escherichia coli. The basis for this switch is unknown. Here, we show that PBP3 protein levels drop drastically when S. Typhimurium senses acidity, high osmolarity and nutrient scarcity, cues that activate virulence functions required for intra-phagosomal survival and proliferation. The protease Prc and the transcriptional regulator OmpR contribute to lower PBP3 levels whereas OmpR stimulates PBP2_SAL /PBP3_SAL production. Surprisingly, despite being essential for division in E. coli, PBP3

SUBMITTER: Lopez-Escarpa D 

PROVIDER: S-EPMC9827838 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature